loading...
Functionalization of Carbon Nanotubes with Antibodies for Breast Cancer Detection Applications
Banff, Alberta, Canada August 25-August 27
DOI Bookmark: http://doi.ieeecomputersociety.org/10.1109/ICMENS.2004.742004 International Conference on MEMS ...
 This Article 
 
PDF
HTML
IEEE Xplore Subscribers
 
 Share 
   
 Bibliographic References 
   
 Add to: 
 
Digg
Furl
Spurl
Blink
Simpy
Google
Del.icio.us
Y!MyWeb
 
 Search 
   
Ranjani Sirdeshmukh, University of Delaware
Kasif Teker, University of Delaware
Balaji Panchapakesan, University of Delaware
We study the effect of functionalization of Carbon Nanotubes (CNTs) with a primary monoclonal mouse immunoglobin G (IgG) specific to the cell-surface receptors of breast cancer cells, and secondary polyclonal goat ant- mouse IgG. The CNTs, in solution with a surfactant (sodium dodecyl benzene sulfonate) were labeled with dihexyloxacarbocyanine iodide (DiOC6), a fluorescent dye, in order to view them with fluorescently labeled antibodies through confocal microscopy. Co-localization for CNTs in combination with the primary antibody conjugated to the secondary was determined to be 90%, whereas CNTs in combination with the secondary antibody and polyethylene glycol (PEG), a polymer used to block CNTs from proteins binding to their surface, was found to be very minimal (0.5%). Preliminary studies on the electrical measurements of the primary mouse IgG incubated with CNTs show a decrease in conductance compared to that of bare CNT field effect transistors (CNTFETs). This observed change in conductance, can eventually be amplified and utilized in applications leading to a full-fledged breast cancer detection system in the future.
Citation:
Ranjani Sirdeshmukh, Kasif Teker, Balaji Panchapakesan, "Functionalization of Carbon Nanotubes with Antibodies for Breast Cancer Detection Applications," icmens, pp.48-53, 2004 International Conference on MEMS, NANO and Smart Systems (ICMENS'04), 2004
Usage of this product signifies your acceptance of the Terms of Use.