In this paper, a cylindrical 3-D B-Spline model of heart’s surfaces is proposed. Since volume is a basic parameter in cardiac image analysis, an effectively integral algorithm for volumetric measurement of the B-Spline surface model represented by matrix forms is presented in this article as well. This method makes the volumetric measurement convenient and its result is more accurate than the results counted by the traditional methods. In order to validate B-Spine surface fitting and advantage of our method for volumetric measurement, several experiments were done. Finally, the results show that the cylindrical B-Spline with a given number of control points can well fit the surfaces of the heart and left ventricle. Experiments also show the algorithm can get satisfactory measurement accuracy and efficiency.