We study relations on trees defined by first-order constraints over a vocabulary that includes the tree extension relation T < T' , holding if and only if every branch of T extends to a branch of T' , unary node-tests, and a binary relation checking if the domains of two trees are equal. We show that from such a formula one can generate a tree automaton that accepts the set of tuples of trees defined by the formula, and conversely that every automaton over tree-tuples is captured by such a formula. We look at the fragment with only extension inequalities and leaf tests, and show that it corresponds to a new class of automata on tree tuples, which is strictly weaker then general tree-tuple automata. We use the automata representations to show separation and expressibility results for formulae in the logic. We then turn to relational calculi over the logic defined here: that is, from constraints we extend to queries that have second-order parameters for a finite set of tree tuples. We give normal forms for queries, and use these to get bounds on the data complexity of query evaluation, showing that while general query evaluation is unbounded within the polynomial hierarchy, generic query evaluation has very low complexity, giving strong bounds on the expressive power of relational calculi with tree extension constraints. We also give normal forms for safe queries in the calculus.